WEKO3
アイテム
Purification and properties of pig kidney D-aspartate oxidase and its utility in analysis of acidic D-amino acids
http://hdl.handle.net/10649/504
http://hdl.handle.net/10649/504e6a1284b-454b-4340-be56-f40c3e2ea056
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
K18_4.pdf (788.5 kB)
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| アイテムタイプ | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-02-15 | |||||
| タイトル | ||||||
| タイトル | Purification and properties of pig kidney D-aspartate oxidase and its utility in analysis of acidic D-amino acids | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | D-Aspartate oxidase | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Pig kidney | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | N-Methyl-D-aspartate | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Acidic D-amino acids | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
山田, 貴裕
× 山田, 貴裕× 長谷川, 晃裕× 松村, 仁志× 内山, 徹× 解良, 芳夫× 山田, 良平 |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2581 | |||||
| 姓名 | ヤマダ, タカヒロ | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2582 | |||||
| 姓名 | ハセガワ, アキヒロ | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2583 | |||||
| 姓名 | マツムラ, ヒロシ | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2584 | |||||
| 姓名 | ウチヤマ, トオル | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2585 | |||||
| 姓名 | ケラ, ヨシオ | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2586 | |||||
| 姓名 | ヤマダ, リョウヘイ | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2587 | |||||
| 姓名 | Yamada, Takahiro | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2588 | |||||
| 姓名 | Hasegawa, Akihiro | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2589 | |||||
| 姓名 | Matsumura, Hitoshi | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2590 | |||||
| 姓名 | Uchiyama, Tooru | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2591 | |||||
| 姓名 | Kera, Yoshio | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2592 | |||||
| 姓名 | Yamada, Ryo-hei | |||||
| 著者所属 | ||||||
| 値 | 長岡技術科学大学生物系 | |||||
| 著者所属 | ||||||
| 値 | 長岡技術科学大学生物系 | |||||
| 著者所属 | ||||||
| 値 | 長岡技術科学大学生物系 | |||||
| 著者所属 | ||||||
| 値 | 長岡技術科学大学生物系 | |||||
| 著者所属 | ||||||
| 値 | 長岡技術科学大学環境・建設系 | |||||
| 著者所属 | ||||||
| 値 | 長岡技術科学大学環境・建設系 | |||||
| 著者所属 | ||||||
| 値 | Dept. of BioEngineering, Nagaoka University of Technology | |||||
| 著者所属 | ||||||
| 値 | Dept. of BioEngineering, Nagaoka University of Technology | |||||
| 著者所属 | ||||||
| 値 | Dept. of BioEngineering, Nagaoka University of Technology | |||||
| 著者所属 | ||||||
| 値 | Dept. of BioEngineering, Nagaoka University of Technology | |||||
| 著者所属 | ||||||
| 値 | Dept. of Civil Environm | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | D-Aspartate oxidase (EC 1.4.3.1) was purified 195-fold from pig kidney cortex. The purified preparation had a specific activity of 5279 nmol/min per mg of protein with D-aspartate as substrate and consisted of two major proteins of 53 kDa and 57 kDa with other minor proteins as indicated by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The molecular mass of the native enzyme was estimated to be 230 kDa by gel filtration. The optimum pH was rather broad ranging from 7.5 to 9.5 with a maximum at 8.5. The optimum temperature was 45\C\, while the stability decreased with the increase in temperature above 30\C\. The enzyme did not follow Michaelis-Menten kinetics probably because of substrate activation. For this reason, the Km values were obtained with higher concentrations of substrates and were 4.77, 1.91 and 51.1 mM for D-aspartate, N-menthyl-D-aspartate and D-glutamate respectively. Of the compounds tested meso-tartrate was the most effective competitive inhibitor with a Ki calue of 1.44mM. To utilize the purified preparation for enzymatic analysis of acidic D-amino acids, various reaction conditions were tested. The reaction systems thus established including those for differential determination of D-aspartate and D-glutamate are described. | |||||
| 書誌情報 |
長岡技術科学大学研究報告 巻 18, p. 19-26, 発行日 1996-12 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0388-5631 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00177120 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 出版者 | ||||||
| 出版者 | 長岡技術科学大学 | |||||
