WEKO3
アイテム
Grafting of polyacrylamide onto silica gel and its application to enzyme immobilization
http://hdl.handle.net/10649/522
http://hdl.handle.net/10649/522c48990a7-eaf4-4de5-bf67-e50a82463324
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
K19_5.pdf (660.3 kB)
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| アイテムタイプ | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-02-15 | |||||
| タイトル | ||||||
| タイトル | Grafting of polyacrylamide onto silica gel and its application to enzyme immobilization | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Polyacrylamide | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Silica gel | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Enzyme | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Glucose Oxidase | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Immobilization | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
下村, 雅人
× 下村, 雅人× 朝倉, 全紀× Wang, Nan× 山内, 健× 宮内, 信之助 |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2776 | |||||
| 姓名 | シモムラ, マサト | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2777 | |||||
| 姓名 | アサクラ, マサキ | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2778 | |||||
| 姓名 | ヤマウチ, タケシ | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2779 | |||||
| 姓名 | ミヤウチ, シンノスケ | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2780 | |||||
| 姓名 | Shimomura, Masato | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2781 | |||||
| 姓名 | Asakura, Masaki | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2782 | |||||
| 姓名 | Yamauchi, Takeshi | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2783 | |||||
| 姓名 | Miyauchi, Shinnosuke | |||||
| 著者所属 | ||||||
| 値 | 長岡技術科学大学生物系 | |||||
| 著者所属 | ||||||
| 値 | 長岡技術科学大学生物系 | |||||
| 著者所属 | ||||||
| 値 | 長岡技術科学大学生物系 | |||||
| 著者所属 | ||||||
| 値 | 長岡技術科学大学生物系 | |||||
| 著者所属 | ||||||
| 値 | 長岡技術科学大学生物系 | |||||
| 著者所属 | ||||||
| 値 | Dept. of BioEngineering, Nagaoka University of Technology | |||||
| 著者所属 | ||||||
| 値 | Dept. of BioEngineering, Nagaoka University of Technology | |||||
| 著者所属 | ||||||
| 値 | Dept. of BioEngineering, Nagaoka University of Technology | |||||
| 著者所属 | ||||||
| 値 | Dept. of BioEngineering, Nagaoka University of Technology | |||||
| 著者所属 | ||||||
| 値 | Dept. of BioEngineering | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Polymerization of acrylamide was carried out in a redox system consisting of ceric iron and mercapto groups introduced onto silica gel. In the course of the polymerization, plyacrylamid was grafted onto the surface of the silica gel. The amount of the grafted polymer reached 130 mg per gram of the silica gel employed in the polymerzation. In order to immobilize glucose oxidase on the silica gel, the coupling reaction between the grafted polymer and the enzyme was attempted in the presence of a condensing agent. It was shown that 4.3 mg of the enzyme was immobilized on 1 g of the polyacrylamid-grafted silica gel, and the enzyme immobilized product had an apparent activity of 50 units/g. The pH-activity and temperature-activity profiles of the immobilized enzyme resembled those of the native enzyme. A kinetic study of the glucose oxidation with the immobilized enzyme allowed the consideration that the enzyme immobilization caused retardation of lactone formation from the enzyme-glucose complex. The immobilized enzyme kept 95% of its original activity in water over a period of 3 months. | |||||
| 書誌情報 |
長岡技術科学大学研究報告 巻 19, p. 37-43, 発行日 1997-12-25 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0388-5631 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00177120 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 出版者 | ||||||
| 出版者 | 長岡技術科学大学 | |||||
