WEKO3
アイテム
Basic studies on spectrophotometric and chemiluminescent determination of D-aspartate using D-aspartate oxidase from Cryptococcus humicolus UJ1
http://hdl.handle.net/10649/545
http://hdl.handle.net/10649/54592072f4c-f411-439b-b9ed-a423c13ed135
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
K20_2.pdf (541.1 kB)
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| アイテムタイプ | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-02-15 | |||||
| タイトル | ||||||
| タイトル | Basic studies on spectrophotometric and chemiluminescent determination of D-aspartate using D-aspartate oxidase from Cryptococcus humicolus UJ1 | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | D-Aspartate determination | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | D-Aspartate oxidase | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Spectrophotometric determination | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Chemiluminescent determination | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
松村, 嘉子
× 松村, 嘉子× 解良, 芳夫× 山田, 良平 |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2928 | |||||
| 姓名 | マツムラ, ヨシコ | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2929 | |||||
| 姓名 | ケラ, ヨシオ | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2930 | |||||
| 姓名 | ヤマダ, リョウヘイ | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2931 | |||||
| 姓名 | Matsumura, Yoshiko | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2932 | |||||
| 姓名 | Kera, Yoshio | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2933 | |||||
| 姓名 | Yamada, Ryo-hei | |||||
| 著者所属 | ||||||
| 値 | 長岡技術科学大学生物系 | |||||
| 著者所属 | ||||||
| 値 | 長岡技術科学大学環境・建設系 | |||||
| 著者所属 | ||||||
| 値 | 長岡技術科学大学環境・建設系 | |||||
| 著者所属 | ||||||
| 値 | Dept. of BioEngineering, Nagaoka University of Technology | |||||
| 著者所属 | ||||||
| 値 | Dept. of Civil and Environmental Engineering, Nagaoka University of Technology | |||||
| 著者所属 | ||||||
| 値 | Dept. of Civil and Environmental Engineering, Nagaoka University of Technology | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | A spectrophotometric method and a chemiluminescent method were studied for determination of minute amounts of D-aspartate using D-aspartate oxidase of the yeast Cryptococcus humicolus UJ1 that catalyzes very specifically the oxidative deamination of D-aspartate. The spectrophometric method was based on the measurement of 2,4-dinitrophenylhydrazones derived from keto acids produced from D-aspartate by the action of the enzyme. Addition of catalase to the enzyme reaction mixture was required to degrade hydrogen peroxide, another reaction product from D-aspartate and oxygen, so that the keto acids would not be decreased by the reaction with the hydrogen peroxide. The detection limit for D-aspartate was 1nmol, and the presence of 100nmol D-glutamate did not affect the detection at all. The chemiluminescent method depended on the measurement of the hydrogen peroxide as a product by the chemiluminescent reaction of hydrogen peroxide with bis (2,4,6-trichlorophenyl) oxalate in the presence of a fluorescent dye, 8-anilinonaphthalene-1-sulfonic acid. The detection limit appeared to be around 20 pmol of D-aspartate, | |||||
| 書誌情報 |
長岡技術科学大学研究報告 巻 20, p. 9-13, 発行日 1999-02-10 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0388-5631 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00177120 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 出版者 | ||||||
| 出版者 | 長岡技術科学大学 | |||||
